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mtor substrates antibody sampler kit 9862 cst  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mtor substrates antibody sampler kit 9862 cst
    <t>PI3K/AKT/mTOR</t> signaling in induced spheroidgenesis. Western blot analysis shows mTOR activation and signaling during induced spheroidgenesis, which parallels increases in HIF-1α levels ( A ). Both mTOR signaling and HIF-1α levels are greater in E-cadherin-positive cell lines than -negative cell lines and are significantly inhibited by rapamycin, but Notch1 signaling is lower ( B , C ). Western blot also shows that PI3K/AKT signaling increases HIF-1α levels more in E-cadherin-positive cell lines than -negative cell lines ( D , E ). This inhibitor does not appreciably affect Notch1 signaling nor alterations in either PHD-2/Egln1 or VHL ( D , E ).
    Mtor Substrates Antibody Sampler Kit 9862 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtor+substrates+antibody+sampler+kit+9862+cst/pmc12730275-27-35-41?v=Cell+Signaling+Technology+Inc
    Average 93 stars, based on 68 article reviews
    mtor substrates antibody sampler kit 9862 cst - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "E-Cadherin Regulates HIF1-α In Vitro in Induced 3D Spheroid Models of Human Breast Cancer Through Both mTOR and Notch1 Signaling"

    Article Title: E-Cadherin Regulates HIF1-α In Vitro in Induced 3D Spheroid Models of Human Breast Cancer Through Both mTOR and Notch1 Signaling

    Journal: Biomedicines

    doi: 10.3390/biomedicines13122890

    PI3K/AKT/mTOR signaling in induced spheroidgenesis. Western blot analysis shows mTOR activation and signaling during induced spheroidgenesis, which parallels increases in HIF-1α levels ( A ). Both mTOR signaling and HIF-1α levels are greater in E-cadherin-positive cell lines than -negative cell lines and are significantly inhibited by rapamycin, but Notch1 signaling is lower ( B , C ). Western blot also shows that PI3K/AKT signaling increases HIF-1α levels more in E-cadherin-positive cell lines than -negative cell lines ( D , E ). This inhibitor does not appreciably affect Notch1 signaling nor alterations in either PHD-2/Egln1 or VHL ( D , E ).
    Figure Legend Snippet: PI3K/AKT/mTOR signaling in induced spheroidgenesis. Western blot analysis shows mTOR activation and signaling during induced spheroidgenesis, which parallels increases in HIF-1α levels ( A ). Both mTOR signaling and HIF-1α levels are greater in E-cadherin-positive cell lines than -negative cell lines and are significantly inhibited by rapamycin, but Notch1 signaling is lower ( B , C ). Western blot also shows that PI3K/AKT signaling increases HIF-1α levels more in E-cadherin-positive cell lines than -negative cell lines ( D , E ). This inhibitor does not appreciably affect Notch1 signaling nor alterations in either PHD-2/Egln1 or VHL ( D , E ).

    Techniques Used: Western Blot, Activation Assay



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    mtor substrates antibody sampler kit 9862 cst  (Cell Signaling Technology Inc)
    93
    Cell Signaling Technology Inc mtor substrates antibody sampler kit 9862 cst
    <t>PI3K/AKT/mTOR</t> signaling in induced spheroidgenesis. Western blot analysis shows mTOR activation and signaling during induced spheroidgenesis, which parallels increases in HIF-1α levels ( A ). Both mTOR signaling and HIF-1α levels are greater in E-cadherin-positive cell lines than -negative cell lines and are significantly inhibited by rapamycin, but Notch1 signaling is lower ( B , C ). Western blot also shows that PI3K/AKT signaling increases HIF-1α levels more in E-cadherin-positive cell lines than -negative cell lines ( D , E ). This inhibitor does not appreciably affect Notch1 signaling nor alterations in either PHD-2/Egln1 or VHL ( D , E ).
    Mtor Substrates Antibody Sampler Kit 9862 Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtor+substrates+antibody+sampler+kit+9862+cst/pmc12730275-27-35-41?v=Cell+Signaling+Technology+Inc
    Average 93 stars, based on 1 article reviews
    mtor substrates antibody sampler kit 9862 cst - by Bioz Stars, 2026-07
    93/100 stars
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    anti tsc2 the mtor pathway antibody sampler kit cell signaling cst 9862t  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc anti tsc2 the mtor pathway antibody sampler kit cell signaling cst 9862t
    E260 inhibits ERK1/2 signaling cascade and mTORC1 activation in PDAC cells. SU.86.86 and PANC-1 cells were left untreated or subjected to 2.5 µM E260 for 48 hr, in MEM supplied with 2mM L-glutamine. Cells were then harvested, and their lysates were resolved in SDS-PAGE and subjected to (A) anti-pospho-ERK1/2 (Thr.202/Tyr204), anti-ERK1/2, <t>anti-phspho-TSC2</t> (Ser 664), anti-TSC, and anti-Tubulin. (B) anti-phospho-mTOR (Ser 2448), anti-mTOR, and anti-Tubulin, in a WB analysis. (C) Asparagine alleviates the death level evoked by E260 in PDAC cells. SU.86.86 cells were left untreated or treated with 2.5 µM E260, in the absence or presence of 4 mM asparagine, for 48 hr. The percentage of viable cells in each sample was determined using an automatic cell counter after the addition of Trypan blue to the sample. Data represent average values of three independent experiments that gave similar results. Standard deviations and P values are presented. (D) Lysates from each sample were resolved in SDS-PAGE and reacted with: anti-phospho-mTOR (Ser 2448), anti-mTOR (left panel), anti- phosphor-S6K (Thr389), anti-S6K (right panel), and anti-Tubulin, in a WB analysis. In each panel, one out of three independent experiments that gave similar results is presented.
    Anti Tsc2 The Mtor Pathway Antibody Sampler Kit Cell Signaling Cst 9862t, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtor+substrates+antibody+sampler+kit+9862+cst/pmc11349523-70-72-79?v=Cell+Signaling+Technology+Inc
    Average 93 stars, based on 1 article reviews
    anti tsc2 the mtor pathway antibody sampler kit cell signaling cst 9862t - by Bioz Stars, 2026-07
    93/100 stars
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    Image Search Results


    PI3K/AKT/mTOR signaling in induced spheroidgenesis. Western blot analysis shows mTOR activation and signaling during induced spheroidgenesis, which parallels increases in HIF-1α levels ( A ). Both mTOR signaling and HIF-1α levels are greater in E-cadherin-positive cell lines than -negative cell lines and are significantly inhibited by rapamycin, but Notch1 signaling is lower ( B , C ). Western blot also shows that PI3K/AKT signaling increases HIF-1α levels more in E-cadherin-positive cell lines than -negative cell lines ( D , E ). This inhibitor does not appreciably affect Notch1 signaling nor alterations in either PHD-2/Egln1 or VHL ( D , E ).

    Journal: Biomedicines

    Article Title: E-Cadherin Regulates HIF1-α In Vitro in Induced 3D Spheroid Models of Human Breast Cancer Through Both mTOR and Notch1 Signaling

    doi: 10.3390/biomedicines13122890

    Figure Lengend Snippet: PI3K/AKT/mTOR signaling in induced spheroidgenesis. Western blot analysis shows mTOR activation and signaling during induced spheroidgenesis, which parallels increases in HIF-1α levels ( A ). Both mTOR signaling and HIF-1α levels are greater in E-cadherin-positive cell lines than -negative cell lines and are significantly inhibited by rapamycin, but Notch1 signaling is lower ( B , C ). Western blot also shows that PI3K/AKT signaling increases HIF-1α levels more in E-cadherin-positive cell lines than -negative cell lines ( D , E ). This inhibitor does not appreciably affect Notch1 signaling nor alterations in either PHD-2/Egln1 or VHL ( D , E ).

    Article Snippet: Antibodies used included the following: Notch Activated Targets Antibody Sampler Kit, #68309, Cell Signaling Technology (CST), Danvers, MA, USA; Hypoxia Pathway Antibody, Sampler Kit, #15792, CST, which include antibodies to HIF-2α, VHL, hydroxy-HIF-1α, and PHD-2/Egln1; mTOR Substrates Antibody Sampler Kit, #9862, CST; and E-cadherin (G-10), #sc-8426, Santa Cruz Biotechnology, Santa Cruz, CA, USA; and Calpain 2 Large Subunit (M-type) (1:1000 dilution, #3195, (CST)).

    Techniques: Western Blot, Activation Assay

    E260 inhibits ERK1/2 signaling cascade and mTORC1 activation in PDAC cells. SU.86.86 and PANC-1 cells were left untreated or subjected to 2.5 µM E260 for 48 hr, in MEM supplied with 2mM L-glutamine. Cells were then harvested, and their lysates were resolved in SDS-PAGE and subjected to (A) anti-pospho-ERK1/2 (Thr.202/Tyr204), anti-ERK1/2, anti-phspho-TSC2 (Ser 664), anti-TSC, and anti-Tubulin. (B) anti-phospho-mTOR (Ser 2448), anti-mTOR, and anti-Tubulin, in a WB analysis. (C) Asparagine alleviates the death level evoked by E260 in PDAC cells. SU.86.86 cells were left untreated or treated with 2.5 µM E260, in the absence or presence of 4 mM asparagine, for 48 hr. The percentage of viable cells in each sample was determined using an automatic cell counter after the addition of Trypan blue to the sample. Data represent average values of three independent experiments that gave similar results. Standard deviations and P values are presented. (D) Lysates from each sample were resolved in SDS-PAGE and reacted with: anti-phospho-mTOR (Ser 2448), anti-mTOR (left panel), anti- phosphor-S6K (Thr389), anti-S6K (right panel), and anti-Tubulin, in a WB analysis. In each panel, one out of three independent experiments that gave similar results is presented.

    Journal: Frontiers in Oncology

    Article Title: Fer governs mTORC1 regulating pathways and sustains viability of pancreatic ductal adenocarcinoma cells

    doi: 10.3389/fonc.2024.1427029

    Figure Lengend Snippet: E260 inhibits ERK1/2 signaling cascade and mTORC1 activation in PDAC cells. SU.86.86 and PANC-1 cells were left untreated or subjected to 2.5 µM E260 for 48 hr, in MEM supplied with 2mM L-glutamine. Cells were then harvested, and their lysates were resolved in SDS-PAGE and subjected to (A) anti-pospho-ERK1/2 (Thr.202/Tyr204), anti-ERK1/2, anti-phspho-TSC2 (Ser 664), anti-TSC, and anti-Tubulin. (B) anti-phospho-mTOR (Ser 2448), anti-mTOR, and anti-Tubulin, in a WB analysis. (C) Asparagine alleviates the death level evoked by E260 in PDAC cells. SU.86.86 cells were left untreated or treated with 2.5 µM E260, in the absence or presence of 4 mM asparagine, for 48 hr. The percentage of viable cells in each sample was determined using an automatic cell counter after the addition of Trypan blue to the sample. Data represent average values of three independent experiments that gave similar results. Standard deviations and P values are presented. (D) Lysates from each sample were resolved in SDS-PAGE and reacted with: anti-phospho-mTOR (Ser 2448), anti-mTOR (left panel), anti- phosphor-S6K (Thr389), anti-S6K (right panel), and anti-Tubulin, in a WB analysis. In each panel, one out of three independent experiments that gave similar results is presented.

    Article Snippet: The following antibodies were used in the current work: affinity purified polyclonal anti-N-terminal Fer (anti-N-Fer, directed toward amino acids 1–189, of Fer), and anti-Fer -SH2 antibodies that were generated in our lab ( , )., anti-ATP5B mitochondrial ATP synthase beta subunit monoclonal antibody (Santa Cruz Biotechnology), anti-phospho-AMPK (Thr172) (Cell Signaling CST-#4188), anti-AMPKα1 (Santa Cruz Biotechnology sc-398861), anti -phospho-Raptor (Ser792) (Cell Signaling CST-2083P), anti-Raptor (Cell Signaling CST-9862T), anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phosphoTSC2 (Ser664), and anti-TSC2 (the mTOR Pathway Antibody Sampler Kit, Cell Signaling CST-9862T), anti-phospho-ERK1/2 (Thr202,Tyr204) (Sigma-Aldrich SAB4301578), anti-ERK1/2 (Abcam ab17942), anti-phospho-MEK1/2 (Ser218/222)- (Santa Cruz Biotechnology sc7995), anti-MEK1/2 (Santa Cruz Biotechnology sc-436), anti-NDUFA9 (Abcam-ab55521), anti-ATP5A (Abcam ab176569), anti-S6K (Abcam- ab186753), anti-phospho-S6K (Thr389) (Abcam ab32359), anti-actin (Santa Cruz Biotechnology sc-8432), and anti-α tubulin antibody (Santa Cruz Biotechnology sc-8035).

    Techniques: Activation Assay, SDS Page

    Knockdown of Fer activates AMPK and downregulates ERK1/2 in PDAC cells. (A) SU.86.86. cells were transfected with control siRNA (siRNAc), or fer -targeting siRNA (siRNA fer ) and incubated in MEM supplied with 2 mM L-glutamine, for 72 hr. Cells were then harvested, and their lysates were resolved in SDS-PAGE and subjected to: anti-Fer (SH2), anti-pospho-ERK1/2 (Thr.202/Tyr204), anti-ERK1/2, anti-phspho-TSC2 (Ser 664), anti-TSC, anti-phospoh-AMPK (Thr172), anti-AMPK, anti-phospho-mTOR (Ser 2448), anti-mTOR, and anti-Tubulin, in a WB analysis. One out of three independent experiments that gave similar results is presented. (B) Summarizing scheme depicting the two regulatory pathways affected by E260 and converging to the downregulation of mTORC1 and mitochondrial function, in PDAC cells.

    Journal: Frontiers in Oncology

    Article Title: Fer governs mTORC1 regulating pathways and sustains viability of pancreatic ductal adenocarcinoma cells

    doi: 10.3389/fonc.2024.1427029

    Figure Lengend Snippet: Knockdown of Fer activates AMPK and downregulates ERK1/2 in PDAC cells. (A) SU.86.86. cells were transfected with control siRNA (siRNAc), or fer -targeting siRNA (siRNA fer ) and incubated in MEM supplied with 2 mM L-glutamine, for 72 hr. Cells were then harvested, and their lysates were resolved in SDS-PAGE and subjected to: anti-Fer (SH2), anti-pospho-ERK1/2 (Thr.202/Tyr204), anti-ERK1/2, anti-phspho-TSC2 (Ser 664), anti-TSC, anti-phospoh-AMPK (Thr172), anti-AMPK, anti-phospho-mTOR (Ser 2448), anti-mTOR, and anti-Tubulin, in a WB analysis. One out of three independent experiments that gave similar results is presented. (B) Summarizing scheme depicting the two regulatory pathways affected by E260 and converging to the downregulation of mTORC1 and mitochondrial function, in PDAC cells.

    Article Snippet: The following antibodies were used in the current work: affinity purified polyclonal anti-N-terminal Fer (anti-N-Fer, directed toward amino acids 1–189, of Fer), and anti-Fer -SH2 antibodies that were generated in our lab ( , )., anti-ATP5B mitochondrial ATP synthase beta subunit monoclonal antibody (Santa Cruz Biotechnology), anti-phospho-AMPK (Thr172) (Cell Signaling CST-#4188), anti-AMPKα1 (Santa Cruz Biotechnology sc-398861), anti -phospho-Raptor (Ser792) (Cell Signaling CST-2083P), anti-Raptor (Cell Signaling CST-9862T), anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phosphoTSC2 (Ser664), and anti-TSC2 (the mTOR Pathway Antibody Sampler Kit, Cell Signaling CST-9862T), anti-phospho-ERK1/2 (Thr202,Tyr204) (Sigma-Aldrich SAB4301578), anti-ERK1/2 (Abcam ab17942), anti-phospho-MEK1/2 (Ser218/222)- (Santa Cruz Biotechnology sc7995), anti-MEK1/2 (Santa Cruz Biotechnology sc-436), anti-NDUFA9 (Abcam-ab55521), anti-ATP5A (Abcam ab176569), anti-S6K (Abcam- ab186753), anti-phospho-S6K (Thr389) (Abcam ab32359), anti-actin (Santa Cruz Biotechnology sc-8432), and anti-α tubulin antibody (Santa Cruz Biotechnology sc-8035).

    Techniques: Knockdown, Transfection, Control, Incubation, SDS Page